Murine model of long-term obstructive jaundice.Īoki, Hiroaki Aoki, Masayo Yang, Jing Katsuta, Eriko Mukhopadhyay, Partha Ramanathan, Rajesh Woelfel, Ingrid A Wang, Xuan Spiegel, Sarah Zhou, Huiping Takabe, Kazuaki The LHML model was identified to be the most feasible model to study the effect of long-term obstructive jaundice. pCL may be an appropriate model for acute inflammation with obstructive jaundice but long term survivors are no longer jaundiced. Conclusion The standard tCL model is too unstable with high mortality for long-term studies. The LHML group developed portal hypertension as well as severe fibrosis by Day 14 in addition to prolonged jaundice. The pCL model demonstrated acute inflammation due to obstructive jaundice 3 days after ligation but jaundice rapidly decreased by Day 7. Compensatory enlargement of the right lobe was observed in both pCL and LMHL models. 19% (3/16) of LMHL mice died however, jaundice continued beyond Day 14, with survival of more than a month. Results 70% (7/10) of tCL mice died by Day 7, whereas majority 67% (10/15) of pCL mice survived with loss of jaundice. Fibrotic change was determined by Masson-Trichrome staining and Collagen expression. Survival was assessed by Kaplan-Meier method. Methods C57BL/6J mice were used for total ligation of the common bile duct (tCL), partial common bile duct ligation (pCL), and ligation of left and median hepatic bile duct with gallbladder removal (LMHL) models. Here, we investigated the characteristics of 3 murine models of obstructive jaundice. Wang, Xuan Spiegel, Sarah Zhou, Huiping Takabe, Kazuakiīackground With the recent emergence of conjugated bile acids as signaling molecules in cancer, a murine model of obstructive jaundice by cholestasis with long-term survival is in need. Murine model of long term obstructive jaundiceĪoki, Hiroaki Aoki, Masayo Yang, Jing Katsuta, Eriko Mukhopadhyay, Partha Ramanathan, Rajesh Woelfel, Ingrid A.
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